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p stat3tyr705  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p stat3tyr705
    P Stat3tyr705, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+stat3tyr705/pm41390680-287-35-37
    Average 86 stars, based on 1 article reviews
    p stat3tyr705 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Pancreatic β cell interleukin-22 receptor subunit alpha 1 deficiency impairs β cell function in type 2 diabetes via cytochrome b5 reductase 3.
    Article Snippet: 5X protein loading buffer (Beyotime Biotechnology, P0286) was added to the residual supernatants and boiled for 20 min. About 30mg of total protein was subjected to sodium dodecyl-sulfate polyacrylamide gel (SDS-PAGE) electrophoresis, and transferred to nitrocellulose membranes (Millipore, Billerica, MA, USA). .. After blocking with 5% BSA for 1.5h, membranes were incubated overnight at 4 C with the following primary antibodies at a dilution of 1:1000: b-Actin (Cell Signaling Technology, 4970), STAT3 (Cell Signaling Technology, 4904), p-STAT3Tyr705 (Cell Signaling Technology, 9131), IL22RA1 (Invitrogen, PA5-19987), c-Jun (Cell Signaling Technology, 9165), p-JunSer73 (Cell Signaling Technology, 9164), CYB5R3 (Proteintech, 10894-1- AP), OXPHOS (Abcam, ab110413), MAFA (Cell Signaling Technology, 79737), PDX1 (Abcam, ab47308), NKX6.1 (Cell Signaling Technology, 54551). .. Next day, membranes were incubated with secondary antibodies at room temperature for 1 h. HRP peroxidase-conjugated anti-mouse IgG (Cell Signaling Technology, 7076) and HRP peroxidase-conjugated anti-rabbit IgG (Cell Signaling Technology, 7074).

    Incubation:

    Article Title: Pancreatic β cell interleukin-22 receptor subunit alpha 1 deficiency impairs β cell function in type 2 diabetes via cytochrome b5 reductase 3.
    Article Snippet: 5X protein loading buffer (Beyotime Biotechnology, P0286) was added to the residual supernatants and boiled for 20 min. About 30mg of total protein was subjected to sodium dodecyl-sulfate polyacrylamide gel (SDS-PAGE) electrophoresis, and transferred to nitrocellulose membranes (Millipore, Billerica, MA, USA). .. After blocking with 5% BSA for 1.5h, membranes were incubated overnight at 4 C with the following primary antibodies at a dilution of 1:1000: b-Actin (Cell Signaling Technology, 4970), STAT3 (Cell Signaling Technology, 4904), p-STAT3Tyr705 (Cell Signaling Technology, 9131), IL22RA1 (Invitrogen, PA5-19987), c-Jun (Cell Signaling Technology, 9165), p-JunSer73 (Cell Signaling Technology, 9164), CYB5R3 (Proteintech, 10894-1- AP), OXPHOS (Abcam, ab110413), MAFA (Cell Signaling Technology, 79737), PDX1 (Abcam, ab47308), NKX6.1 (Cell Signaling Technology, 54551). .. Next day, membranes were incubated with secondary antibodies at room temperature for 1 h. HRP peroxidase-conjugated anti-mouse IgG (Cell Signaling Technology, 7076) and HRP peroxidase-conjugated anti-rabbit IgG (Cell Signaling Technology, 7074).

    Article Title: The systemic lupus erythematosus-associated NCF1 90H allele synergizes with viral infection to cause mouse lupus but also limits virus spread.
    Article Snippet: .. Immunofluorescence BMDMs from the naïve and IFN-α stimulated mice were cultured in confocal dishes, fixed with 4% paraformaldehyde, blocked with 10% FCS/PBS buffer, and then incubated with the primary antibodies: mouse anti-p47phox (D-10) antibodies (Santa Cruz Biotechnology, sc17845), or STAT1 (CST, clone: D1K9Y, 65748), p-STAT1Tyr701 (CST, clone: 58D6, 88845), STAT3 (CST, clone: 79D7, 4904), and p-STAT3Tyr705 (CST, clone: Tyr705, 9131) specific rabbit antibodies (1:1000 dilutions). .. Subsequently, cells were incubated with either goat anti-mouse IgG (H + L) (1:1000 dilutions; CST, 4408) or goat anti-rabbit IgG (H+ L)Alexa Fluor 488 (1:1000 dilutions; CST, 4412), followed by counterstaining with 4’, 6-diamidino-2-phenylindole DAPI (Vector, CA) and dried for 30min before scanning under a confocal microscope (LSM880 with Airyscan, CarlZeiss, Germany).

    Article Title: Interleukin-6 Modulates the Expression and Function of HCN Channels: A Link Between Inflammation and Atrial Electrogenesis
    Article Snippet: Subsequently, 20 μg of proteins were separated on 4–20% SDS-PAGE (Thermo Fisher Scientific, Carlsbad, CA, USA) and transferred into PVDF membranes (60 min at 398 mA) using standard procedures. .. Blots were incubated overnight at 4 °C with a specific antibody against HCN1 (rabbit, #APC-056, Lot APC056AG0740, Alomone Labs, Jerusalem, Israel), HCN2 (rabbit, #APC-030, Lot APC030AG0940, Alomone Labs, Jerusalem, Israel), HCN4 (rabbit, #APC-052, Lot APC052AG11120, Alomone Labs, Jerusalem, Israel), MiRP1 (rabbit, #APC-054, Lot AN-03, Alomone Labs, Jerusalem, Israel), p -STAT3Tyr705 (mouse, #4113S, Lot 6, Cell Signaling Technology, Leiden, The Netherland), STAT3 (rabbit, #4904S, Lot 7, Cell Signaling Technology, Leiden, The Netherland), and GAPDH (rabbit, #5174S, Lot 8, Cell Signaling Technology, Leiden, The Netherland). ..

    Immunofluorescence:

    Article Title: The systemic lupus erythematosus-associated NCF1 90H allele synergizes with viral infection to cause mouse lupus but also limits virus spread.
    Article Snippet: .. Immunofluorescence BMDMs from the naïve and IFN-α stimulated mice were cultured in confocal dishes, fixed with 4% paraformaldehyde, blocked with 10% FCS/PBS buffer, and then incubated with the primary antibodies: mouse anti-p47phox (D-10) antibodies (Santa Cruz Biotechnology, sc17845), or STAT1 (CST, clone: D1K9Y, 65748), p-STAT1Tyr701 (CST, clone: 58D6, 88845), STAT3 (CST, clone: 79D7, 4904), and p-STAT3Tyr705 (CST, clone: Tyr705, 9131) specific rabbit antibodies (1:1000 dilutions). .. Subsequently, cells were incubated with either goat anti-mouse IgG (H + L) (1:1000 dilutions; CST, 4408) or goat anti-rabbit IgG (H+ L)Alexa Fluor 488 (1:1000 dilutions; CST, 4412), followed by counterstaining with 4’, 6-diamidino-2-phenylindole DAPI (Vector, CA) and dried for 30min before scanning under a confocal microscope (LSM880 with Airyscan, CarlZeiss, Germany).

    Cell Culture:

    Article Title: The systemic lupus erythematosus-associated NCF1 90H allele synergizes with viral infection to cause mouse lupus but also limits virus spread.
    Article Snippet: .. Immunofluorescence BMDMs from the naïve and IFN-α stimulated mice were cultured in confocal dishes, fixed with 4% paraformaldehyde, blocked with 10% FCS/PBS buffer, and then incubated with the primary antibodies: mouse anti-p47phox (D-10) antibodies (Santa Cruz Biotechnology, sc17845), or STAT1 (CST, clone: D1K9Y, 65748), p-STAT1Tyr701 (CST, clone: 58D6, 88845), STAT3 (CST, clone: 79D7, 4904), and p-STAT3Tyr705 (CST, clone: Tyr705, 9131) specific rabbit antibodies (1:1000 dilutions). .. Subsequently, cells were incubated with either goat anti-mouse IgG (H + L) (1:1000 dilutions; CST, 4408) or goat anti-rabbit IgG (H+ L)Alexa Fluor 488 (1:1000 dilutions; CST, 4412), followed by counterstaining with 4’, 6-diamidino-2-phenylindole DAPI (Vector, CA) and dried for 30min before scanning under a confocal microscope (LSM880 with Airyscan, CarlZeiss, Germany).



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